The film confronted the issue of abortion, a sensitive subject in American culture, and won plaudits as well as criticism from members of both the pro-life and pro-choice communities. In the film, the protagonist Juno learns she is pregnant, and she must decide whether to have an abortion or to have the child.

Leah: Pleease, Juno, please!
Juno MacGuff: Well, no, but I want Mark and Vanessa to be suprised and if you tell me I'll just, like, ruin everything.
Bren: What's that supposed to mean?
Ultrasound Technician: I just see a lot of teenage mothers come through here and it's obviously a poisonous environment to raise a baby in.
Juno MacGuff: How do you know I'm so poisonous? What if these adoptive parents turn out to be, like, evil molesters.
Leah: Or, like, stage parents.
Bren: They could be utterly negligent. Maybe they'll do a far shittier job of raising a kid than my dumbass step-daughter would. Have you considered that?
Bren: What is your job title exactly?
Bren: Well, I'm a nail technician and I think we both ought to just stick to what we know.
Bren: Oh, you think you're so special because you get to play Picture Pages up there? Well, my five year old daughter could do that and let me tell you, she's not the brightest bulb in the tanning bed. So why don't you go back to night school in Mantino and learn a real trade.